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KMID : 0861020080230040021
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Korea Journal of Herbology
2008 Volume.23 No. 4 p.21 ~ p.29
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A Study of Morphology and Pattern Analysis in Magnoliae Cortex
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Lee Guem-San
Kim Jung-Hoon
Choi Go-Ya
Kang Dae-Hoon
Hwang Sung-Yeon
Jeong Seung-Il
Kim Hong-Jun
Ju Young-Sung
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Abstract
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Objectives: To determine the standards for discrimination of Magnoliae Cortex, the experiment of specific external-internal characters and the physicochemical pattern analysis were performed.
Methods: External characteristics was observed using a stereoscope. Paraffin-mediated sectioned materials were stained by Ju’s method. Physicochemical patterns of materials were analyzed using HPLC.
Results: 1. Botanical characteristics: Magnolia officinalis had one seed and a white flower, while M. obovata had two seeds and a white flower. Machilus thunbergii had berry and spherical fruits and yellowish green panicles. 2. External characteristics: M. officinalis and M obovata were dark and thick. M. officinalis was gray brown and greasy while M. obovata was light-gray, less oily and smoothly sectioned. Machilus thunbergii was thin and relatively light or yellow-brown, coarsely sectioned and faintly specific scents. 3. Internal characteristics: The bast parts of M. officinalis and M. obovata were commonly wider than Machilus thunbergii The cork cortex of M. officinalis was cell layers with many oil cells, while that of M. obovata was cell layers with less oil cells. Machilus thunbergii’s xylem which consisted of ring-shaped cambium at 1st and 2nd part was occupied in large portion. 4. Physicochemical pattern: Both M. officinalis and M. obovata involved honokiol and magnolol. All kinds of M. officinalis involved Magnatriol B but one kind of M. ovobata and all of Machilus thunbergii didn’t. Machilus thunbergii showed different pattern of chromatogram from that of 2 species above.
Conclusions: These results could be used as standards for discrimination of Magnoliae Cortex and as the method of objectification in medicinal herbs giving the basic resource for bioactivity research.
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KEYWORD
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Magnoliae Cortex, Magnolia officinalis, Machilus thunbergii, morphology, HPLC
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